4KYN
Crystal structure of odorant binding protein 48 from Anopheles gambiae at 3.3 Angstrom resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MAX II BEAMLINE I911-3 |
| Synchrotron site | MAX II |
| Beamline | I911-3 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-02-23 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 32 |
| Unit cell lengths | 52.800, 52.800, 247.860 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 44.967 - 3.300 |
| R-factor | 0.2092 |
| Rwork | 0.209 |
| R-free | 0.24300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4ij7 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.475 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.8.2_1309) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.730 | 3.480 |
| High resolution limit [Å] | 3.300 | 3.300 |
| Rmerge | 0.112 | 0.477 |
| Number of reflections | 11606 | |
| <I/σ(I)> | 6.7 | 2.3 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 4 | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | 4.0 M sodium formate, pH 7, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
