4KYK
Crystal structure of mouse glyoxalase I complexed with indomethacin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SEALED TUBE |
| Source details | OXFORD DIFFRACTION ENHANCE ULTRA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-09-08 |
| Detector | OXFORD ONYX CCD |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 41.865, 65.396, 64.912 |
| Unit cell angles | 90.00, 101.39, 90.00 |
Refinement procedure
| Resolution | 22.779 - 2.000 |
| R-factor | 0.2018 |
| Rwork | 0.199 |
| R-free | 0.25730 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.222 |
| Data reduction software | CrysalisPro |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 22.810 | 2.071 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.129 | 0.595 |
| Number of reflections | 23020 | |
| <I/σ(I)> | 1.78 | |
| Completeness [%] | 98.8 | 97.43 |
| Redundancy | 4.7 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.8 | 289 | 30% PEG 2000, 50mM MES, 0.1M NaCl, pH 5.8, VAPOR DIFFUSION, HANGING DROP, temperature 289K |
