4KYE
Partial Structure of the C-terminal domain of the HPIV4B phosphoprotein, fused to MBP.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX1 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-11-28 |
| Detector | ADSC QUANTUM 210r |
| Wavelength(s) | 0.953695 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 165.782, 44.866, 56.366 |
| Unit cell angles | 90.00, 101.06, 90.00 |
Refinement procedure
| Resolution | 34.950 - 2.600 |
| R-factor | 0.19583 |
| Rwork | 0.193 |
| R-free | 0.24629 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.555 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.950 | 2.660 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Number of reflections | 12667 | |
| <I/σ(I)> | 7.1 | 1.4 |
| Completeness [%] | 99.1 | 95.7 |
| Redundancy | 7.2 | 6.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.7 | 277.15 | 24 %(w/v) PEG 8000, 0.2 M Bis-tris/HCl, 0.5 M Ammonium nitrate , pH 6.7, VAPOR DIFFUSION, SITTING DROP, temperature 277.15K |
