4KYD
Partial Structure of the C-terminal domain of the HPIV4B phosphoprotein, fused to MBP.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 110 |
| Detector technology | IMAGE PLATE |
| Collection date | 2001-09-23 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.54179 |
| Spacegroup name | H 3 |
| Unit cell lengths | 142.830, 142.830, 114.312 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 38.100 - 2.210 |
| R-factor | 0.17631 |
| Rwork | 0.174 |
| R-free | 0.22167 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB accession code 1HSJ |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.387 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.100 | 2.250 |
| High resolution limit [Å] | 2.210 | 2.210 |
| Number of reflections | 43158 | |
| <I/σ(I)> | 4.3 | 1.7 |
| Completeness [%] | 99.1 | 82.1 |
| Redundancy | 9.5 | 6.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.3 | 277.15 | 24 %(w/v) PEG 2000 0.2 M MOPS/KOH, pH 7.30, VAPOR DIFFUSION, SITTING DROP, temperature 277.15K |
