4KYC
Structure of the C-terminal domain of the Menangle virus phosphoprotein, fused to MBP.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
Synchrotron site | Australian Synchrotron |
Beamline | MX2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-08-15 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.9537 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 77.869, 77.869, 197.210 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 36.760 - 1.950 |
R-factor | 0.18635 |
Rwork | 0.185 |
R-free | 0.21254 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | PDB accession 1HSJ |
RMSD bond length | 0.011 |
RMSD bond angle | 1.384 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 36.760 | 1.980 |
High resolution limit [Å] | 1.950 | 1.950 |
Number of reflections | 44924 | |
<I/σ(I)> | 10.6 | 2.7 |
Completeness [%] | 99.8 | 100 |
Redundancy | 12.8 | 13.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 9.1 | 291.15 | 24 %(w/v) PEG8000, 0.2 M Boric acid/KOH, pH 9.1, VAPOR DIFFUSION, SITTING DROP, temperature 291.15K |