4KY9

Structural and Functional Analysis of a Putative Substrate Access Tunnel in the Cytosolic Domain of Human Anion Exchanger 1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	CCD
Collection date	2012-04-24
Detector	ADSC QUANTUM 315
Wavelength(s)	0.97918
Spacegroup name	P 21 21 21
Unit cell lengths	69.905, 80.700, 104.278
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.790 - 2.230
R-factor	0.1871
Rwork	0.185
R-free	0.22340
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.259
Refinement software	PHENIX (1.8.2_1309)

Data quality characteristics

	Overall
Low resolution limit [Å]	43.790
High resolution limit [Å]	2.230
Number of reflections	32556

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	298	100 mM BisTris pH 6.5, 18% PEG 3350, with 0.5% (w/v) octylglucoside, vapor diffusion, sitting drop, temperature 298K