4KY6
Crystal structure of Staphylococcal nuclease variant Delta+PHS L25D at pH 6 and cryogenic temperature
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SEALED TUBE |
| Source details | OTHER |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2013-05-13 |
| Detector | APEX II CCD |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 31.060, 60.426, 38.185 |
| Unit cell angles | 90.00, 94.16, 90.00 |
Refinement procedure
| Resolution | 32.220 - 1.700 |
| R-factor | 0.1954 |
| Rwork | 0.194 |
| R-free | 0.22700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3bdc |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.996 |
| Data reduction software | APEX (II) |
| Phasing software | PHASER |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.780 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.031 | 0.274 |
| Number of reflections | 15570 | |
| <I/σ(I)> | 19.35 | 3.39 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 11.55 | 7.77 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 277 | 21% MPD, 25 mM potassium phosphate, calcium chloride, pdTp, pH 6, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
