4KY0

Crystal structure of a substrate-free glutamate transporter homologue from Thermococcus kodakarensis

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-04-22
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97625
Spacegroup name	P 32 2 1
Unit cell lengths	117.570, 117.570, 309.510
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	48.000 - 3.000
R-factor	0.215
Rwork	0.212
R-free	0.26630
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2nwx
RMSD bond length	0.010
RMSD bond angle	1.510
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.8.2_1309))

Data quality characteristics

	Overall
Low resolution limit [Å]	48.000
High resolution limit [Å]	3.000
Number of reflections	50511
Completeness [%]	99.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	277	25% PEG4000, 100 mM Tris/Bicine, 60 mM CaCl2/MgCl2, 5% OG, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K