4KXT

Structure of human ARGONAUTE1 in complex with guide RNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-04-15
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97920
Spacegroup name	P 21 21 21
Unit cell lengths	69.879, 100.249, 136.701
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.863 - 2.294
R-factor	0.1774
Rwork	0.175
R-free	0.22820
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.165
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.8.2_1309))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.380
High resolution limit [Å]	2.294	2.294
Number of reflections	43638
Completeness [%]	99.9	99.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION	8	293	50 mM Tris-HCl pH 8.0, 50 mM sodium chloride, 5 mM magnesium chloride, 1 mM dithiothreitol, 50% glycerol, EVAPORATION, temperature 293K