4KXN
Crystal structure of DNPH1 (RCL) with kinetine riboside monophosphate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-2 |
| Synchrotron site | ESRF |
| Beamline | ID23-2 |
| Detector technology | CCD |
| Collection date | 2011-02-18 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.87260 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 32.238, 95.449, 79.288 |
| Unit cell angles | 90.00, 101.56, 90.00 |
Refinement procedure
| Resolution | 23.860 - 1.900 |
| R-factor | 0.2348 |
| Rwork | 0.232 |
| R-free | 0.28600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4fyi |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.896 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.970 | 2.000 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.086 | 0.356 |
| Number of reflections | 36476 | |
| <I/σ(I)> | 7.9 | 2.1 |
| Completeness [%] | 98.6 | 96.6 |
| Redundancy | 2.2 | 2.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.6 | 291 | 100 MM TRIS, 1.2 M AMMONIUM SULPHATE, 20MM MGCL2, PH 7.6, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
