4KXI
Crystallographic study of the complex of Ni(II) Schiff base complex and HEW Lysozyme
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2012-02-09 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 77.589, 77.589, 36.390 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 25.110 - 2.000 |
| R-factor | 0.242 |
| Rwork | 0.242 |
| R-free | 0.25600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 193l |
| RMSD bond length | 0.047 |
| RMSD bond angle | 2.500 |
| Data reduction software | AUTOMAR |
| Phasing software | MOLREP |
| Refinement software | CNS (1.3) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | |
| High resolution limit [Å] | 2.000 | 2.000 |
| Number of reflections | 7791 | |
| Completeness [%] | 98.5 | 98.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.8 | 293 | Ni-Schiff-base solution , pH 4.8, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
