4KX4
Crystal structure of Escherichia coli glutaredoxin 2 complex with glutathione
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 32-ID |
| Synchrotron site | APS |
| Beamline | 32-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 50.100, 50.100, 152.470 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 22.469 - 1.600 |
| R-factor | 0.165 |
| Rwork | 0.164 |
| R-free | 0.19350 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.235 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | PHENIX ((phenix.refine: 1.8.2_1309)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 22.470 | 1.690 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.060 | 0.100 |
| Number of reflections | 28685 | |
| <I/σ(I)> | 20.6 | 4.7 |
| Completeness [%] | 94.8 | 67 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.9 | 277 | 4 mM glutathione, 20 mM sodium arsenate, 100 mM sodium citrate, 200 mM ammonium acetate and 30 % PEG 4000, pH 5.9, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
