4KW8
Crystal Structure of Green Fluorescent Protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-B |
| Synchrotron site | APS |
| Beamline | 23-ID-B |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-10-25 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.37645 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 51.623, 63.021, 66.347 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 45.693 - 2.459 |
| R-factor | 0.1598 |
| Rwork | 0.152 |
| R-free | 0.22500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.514 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER (2.5.2) |
| Refinement software | PHENIX (1.8.1_1168) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.540 |
| High resolution limit [Å] | 2.450 | 5.280 | 2.450 |
| Rmerge | 0.174 | 0.106 | 0.889 |
| Number of reflections | 8306 | ||
| <I/σ(I)> | 6.4 | ||
| Completeness [%] | 100.0 | 100 | 100 |
| Redundancy | 6.9 | 6.3 | 6.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | hanging drop | 8.2 | 294 | 50 mM HEPES pH 8.2, 50 mM MgCl2, 22% PEG4000, hanging drop, temperature 294K |
