4KW4
Crystal Structure of Green Fluorescent Protein
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-BM |
Synchrotron site | APS |
Beamline | 22-BM |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-08-20 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 1.0000 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 72.904, 72.904, 113.973 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 23.054 - 1.746 |
R-factor | 0.1723 |
Rwork | 0.170 |
R-free | 0.20450 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.011 |
RMSD bond angle | 1.399 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASER (2.5.0) |
Refinement software | PHENIX (1.8.2_1309) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 1.810 |
High resolution limit [Å] | 1.746 | 3.770 | 1.746 |
Rmerge | 0.088 | 0.031 | 0.822 |
Number of reflections | 31923 | ||
<I/σ(I)> | 8.7 | ||
Completeness [%] | 99.8 | 99.2 | 100 |
Redundancy | 8.3 | 8.8 | 7.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | hanging drop | 8.2 | 294 | 50 mM HEPES pH 8.2, 50 mM MgCl2, 22% PEG4000, hanging drop, temperature 294K |