4KVX

Crystal structure of Naa10 (Ard1) bound to AcCoA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X29A
Synchrotron site	NSLS
Beamline	X29A
Temperature [K]	100
Detector technology	CCD
Collection date	2011-01-01
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9795
Spacegroup name	P 1 21 1
Unit cell lengths	40.974, 64.833, 60.723
Unit cell angles	90.00, 97.55, 90.00

Refinement procedure

Resolution	28.542 - 2.000
R-factor	0.1792
Rwork	0.177
R-free	0.21730
Structure solution method	SAD
Starting model (for MR)	3tfy
RMSD bond length	0.009
RMSD bond angle	1.257
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.8_1069))

Data quality characteristics

	Overall
Low resolution limit [Å]	30.000
High resolution limit [Å]	2.000
Number of reflections	41318

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	293	Protein buffer: 25 mM sodium citrate monobasic, 200 mM NaCl, 4 mM DTT Crystallization well: 0.1 M Bis-tris, 14% Peg 3350, 18% glycerol, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K