4KVU

Crystal structure of a 6-helix coiled coil CC-Hex-L17C-W224BF

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04-1
Synchrotron site	Diamond
Beamline	I04-1
Temperature [K]	68
Detector technology	PIXEL
Collection date	2012-12-04
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.92
Spacegroup name	F 2 2 2
Unit cell lengths	57.010, 60.980, 125.440
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.695 - 1.800
R-factor	0.1954
Rwork	0.194
R-free	0.21580
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.017
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.8.2_1309))

Data quality characteristics

	Overall
Low resolution limit [Å]	31.360
High resolution limit [Å]	1.800
Number of reflections	19390
Completeness [%]	100.0

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	291.15	0.1 M MOPS, 0.1 M magnesium acetate with 12 % w/v PEG 8000, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 291.15K