4KVS
Crystal Structure of Prochlorococcus marinus aldehyde-deformylating oxygenase (mutant A134F)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-09-30 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 77.060, 77.060, 115.860 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 54.490 - 1.670 |
| R-factor | 0.1796 |
| Rwork | 0.179 |
| R-free | 0.18550 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.031 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.8.1_1168)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 54.490 | 1.730 |
| High resolution limit [Å] | 1.670 | 1.670 |
| Number of reflections | 41207 | |
| Completeness [%] | 99.98 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.4 | 277 | 0.1 M SPG buffer, and 25 % w/v PEG 1500 Drop size 200nl plus 200nl , pH 7.4, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
