4KVN
Crystal structure of Fab 39.29 in complex with Influenza Hemagglutinin A/Perth/16/2009 (H3N2)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08ID-1 |
| Synchrotron site | CLSI |
| Beamline | 08ID-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-09-30 |
| Detector | RAYONIX MX-300 |
| Wavelength(s) | 0.979 |
| Spacegroup name | I 21 3 |
| Unit cell lengths | 204.370, 204.370, 204.370 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 64.630 - 3.100 |
| R-factor | 0.2046 |
| Rwork | 0.202 |
| R-free | 0.24840 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3sdy |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.140 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.11.4) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 72.260 | 3.270 |
| High resolution limit [Å] | 3.100 | 3.100 |
| Rmerge | 0.117 | 0.833 |
| Number of reflections | 25898 | |
| <I/σ(I)> | 16.4 | 3.3 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 11.2 | 11.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.2 | 293 | 0.1M Phosphate/Citrate, 40% PEG 300, and 0.7% 1-butanol, pH 4.2, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
