4KVH
Crystal structure of ketosteroid isomerase fold protein Hmuk_0747
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-12-18 |
| Detector | ADSC QUANTUM 210r |
| Wavelength(s) | 0.91951 |
| Spacegroup name | P 63 2 2 |
| Unit cell lengths | 91.785, 91.785, 77.963 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 28.050 - 1.610 |
| R-factor | 0.1576 |
| Rwork | 0.156 |
| R-free | 0.18070 |
| Structure solution method | SAD |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.434 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.640 |
| High resolution limit [Å] | 1.610 | 1.610 |
| Rmerge | 0.070 | |
| Number of reflections | 25722 | |
| <I/σ(I)> | 48.3 | |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 20.4 | 16.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 297 | 0.2M Magnesium chloride, 0.1M Sodium cacodylate, 50% PEG 200, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 297K |
