4KVB
Thermus thermophilus HB27 30S ribosomal subunit lacking ribosomal protein S17
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X25 |
| Synchrotron site | NSLS |
| Beamline | X25 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-03-01 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.1 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 397.316, 397.316, 215.505 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.915 - 4.198 |
| R-factor | 0.2192 |
| Rwork | 0.217 |
| R-free | 0.25760 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4ji5 |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.500 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | PHENIX (dev_1370) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 4.230 |
| High resolution limit [Å] | 3.993 | 3.993 |
| Number of reflections | 145334 | |
| Completeness [%] | 92.6 | 93.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | HANGING DROP | 6.5 | 277 | MPD, pH 6.5, HANGING DROP, temperature 277K |
