4KVA
GTPase domain of Septin 10 from Schistosoma mansoni in complex with GTP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-05-24 |
| Detector | PSI PILATUS 2M |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 160.800, 47.040, 95.480 |
| Unit cell angles | 90.00, 112.61, 90.00 |
Refinement procedure
| Resolution | 74.220 - 2.140 |
| R-factor | 0.187 |
| Rwork | 0.187 |
| R-free | 0.22100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | SCHISTOSOMA MANSONI SEPTIN 10 GTPASE DOMAIN |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.592 |
| Data scaling software | SCALA (3.3.20) |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.8.2_1309) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 74.221 | 2.200 |
| High resolution limit [Å] | 2.140 | 2.140 |
| Rmerge | 0.785 | |
| Number of reflections | 36348 | |
| <I/σ(I)> | 9.9 | 0.9 |
| Completeness [%] | 99.0 | 99.5 |
| Redundancy | 4.8 | 5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.3 | 293 | 0.2 M sodium acetate, 25% PEG 3350, pH 7.3, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
