4KV9
GTPase domain of Septin 10 from Schistosoma mansoni in complex with GDP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I24 |
Synchrotron site | Diamond |
Beamline | I24 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2012-03-15 |
Detector | PSI PILATUS 6M |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 160.900, 45.050, 95.490 |
Unit cell angles | 90.00, 112.17, 90.00 |
Refinement procedure
Resolution | 26.170 - 1.930 |
R-factor | 0.185 |
Rwork | 0.184 |
R-free | 0.21700 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2qnr |
RMSD bond length | 0.009 |
RMSD bond angle | 1.185 |
Data scaling software | SCALA (3.3.20) |
Phasing software | PHASER |
Refinement software | PHENIX (1.8.2_1309) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 26.170 | 1.980 |
High resolution limit [Å] | 1.930 | 1.930 |
Rmerge | 0.633 | |
Number of reflections | 47003 | |
<I/σ(I)> | 10 | 1.2 |
Completeness [%] | 97.6 | 96.3 |
Redundancy | 4 | 3.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 7.3 | 293 | 0.2 M sodium acetate, 25% PEG 3350, pH 7.3, VAPOR DIFFUSION, HANGING DROP, temperature 293K |