4KV7
The crystal structure of a possible leucine/isoleucine/valine-binding protein from Rhodopirellula baltica SH 1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-12-18 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97895 |
| Spacegroup name | P 1 |
| Unit cell lengths | 37.984, 44.483, 57.913 |
| Unit cell angles | 76.22, 88.65, 77.54 |
Refinement procedure
| Resolution | 22.752 - 1.200 |
| R-factor | 0.1489 |
| Rwork | 0.148 |
| R-free | 0.16270 |
| Structure solution method | SAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.122 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | SHELXD |
| Refinement software | PHENIX ((phenix.refine: 1.8.1_1168)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 22.800 | 1.210 |
| High resolution limit [Å] | 1.200 | 1.200 |
| Rmerge | 0.072 | 0.242 |
| Number of reflections | 105324 | |
| <I/σ(I)> | 27 | 2.6 |
| Completeness [%] | 93.8 | 83.7 |
| Redundancy | 1.9 | 1.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 297 | 0.15M DL-Malic Acid, 20%(w/v) PEG3350, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 297K |
