4KV3
Ubiquitin-like domain of the Mycobacterium tuberculosis type VII secretion system protein EccD1 as maltose-binding protein fusion
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-08-16 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 1.0 |
Spacegroup name | P 65 |
Unit cell lengths | 125.680, 125.680, 124.490 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 49.910 - 2.200 |
R-factor | 0.1649 |
Rwork | 0.163 |
R-free | 0.20470 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1anf |
RMSD bond length | 0.009 |
RMSD bond angle | 1.319 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER (2.3.0) |
Refinement software | REFMAC |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 2.260 | ||
High resolution limit [Å] | 2.200 | 9.840 | 2.200 |
Rmerge | 0.105 | 1.097 | |
Number of reflections | 56556 | 654 | 4161 |
<I/σ(I)> | 17.71 | 54.41 | 2.88 |
Completeness [%] | 100.0 | 99.1 | 100 |
Redundancy | 11.5 | 11.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 295 | 0.1M HEPES PH 7.5, 1.4M SODIUM CITRATE, vapor diffusion, sitting drop, temperature 295K |