4KUO
A superfast recovering full-length LOV protein from the marine phototrophic bacterium Dinoroseobacter shibae (Photoexcited state)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-10-28 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.95372 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 90.780, 30.936, 49.538 |
| Unit cell angles | 90.00, 113.09, 90.00 |
Refinement procedure
| Resolution | 26.138 - 2.000 |
| R-factor | 0.1643 |
| Rwork | 0.163 |
| R-free | 0.18240 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4kuk |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.766 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.8.2_1309)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 26.138 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Number of reflections | 8206 | |
| <I/σ(I)> | 16.3 | 10.4 |
| Completeness [%] | 93.5 | 98.15 |
| Redundancy | 3.7 | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5 | 292.15 | 0.3 M magnesium chloride, 8% PGA-LM, 0.1 M sodium acetate, pH 5.0, VAPOR DIFFUSION, SITTING DROP, temperature 292.15K |
