4KUK
A superfast recovering full-length LOV protein from the marine phototrophic bacterium Dinoroseobacter shibae (Dark state)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-1 |
Synchrotron site | ESRF |
Beamline | ID14-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-07-26 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 0.9334 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 89.978, 30.595, 49.332 |
Unit cell angles | 90.00, 113.03, 90.00 |
Refinement procedure
Resolution | 45.399 - 1.500 |
R-factor | 0.1323 |
Rwork | 0.130 |
R-free | 0.17890 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2v0u |
RMSD bond length | 0.012 |
RMSD bond angle | 1.330 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: 1.8.2_1309)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 45.400 | 1.530 |
High resolution limit [Å] | 1.500 | 1.500 |
Rmerge | 0.203 | |
Number of reflections | 19912 | |
<I/σ(I)> | 19.7 | 4.3 |
Completeness [%] | 99.0 | 89.3 |
Redundancy | 3.3 | 2.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5 | 292.15 | 0.3 M magnesium chloride, 8% PGA-LM, 0.1 M sodium acetate, pH 5.0, VAPOR DIFFUSION, SITTING DROP, temperature 292.15K |