4KTP
Crystal structure of 2-O-alpha-glucosylglycerol phosphorylase in complex with glucose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE AR-NE3A |
| Synchrotron site | Photon Factory |
| Beamline | AR-NE3A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-11-26 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 1.00000 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 206.076, 76.959, 145.766 |
| Unit cell angles | 90.00, 128.39, 90.00 |
Refinement procedure
| Resolution | 19.940 - 1.900 |
| R-factor | 0.1718 |
| Rwork | 0.170 |
| R-free | 0.20610 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.025 |
| RMSD bond angle | 2.356 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.930 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Number of reflections | 140301 | |
| <I/σ(I)> | 16.3 | 3.2 |
| Completeness [%] | 99.5 | 100 |
| Redundancy | 3.7 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 28% PEG 400, 0.1M HEPES, 0.2M calcium chloride, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293.0K |
