4KT3

Structure of a type VI secretion system effector-immunity complex from Pseudomonas protegens

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.3
Synchrotron site	ALS
Beamline	5.0.3
Temperature [K]	100
Detector technology	CCD
Collection date	2013-01-26
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.976
Spacegroup name	P 21 21 21
Unit cell lengths	40.112, 81.515, 86.453
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.227 - 1.436
R-factor	0.1497
Rwork	0.149
R-free	0.17500
Structure solution method	SAD
RMSD bond length	0.007
RMSD bond angle	1.105
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX (AutoSol)
Refinement software	PHENIX ((phenix.refine: 1.8.1_1168))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	59.310	1.470
High resolution limit [Å]	1.436	1.436
Number of reflections	52547
Completeness [%]	99.9	99.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	297	0.1 M Tris-HCl pH 8.5, 0.2 M calcium chloride, 30% (w/v) PEG4000, VAPOR DIFFUSION, SITTING DROP, temperature 297K