4KT1

Complex of R-spondin 1 with LGR4 extracellular domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2012-07-01
Detector	RIGAKU
Wavelength(s)	0.97
Spacegroup name	P 32
Unit cell lengths	91.376, 91.376, 87.273
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	24.671 - 2.497
R-factor	0.1626
Rwork	0.160
R-free	0.20860
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3zyo
RMSD bond length	0.007
RMSD bond angle	1.159
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASES
Refinement software	PHENIX ((phenix.refine: 1.8.1_1168))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.560
High resolution limit [Å]	2.497	2.500
Number of reflections	28261
Completeness [%]	99.9	99.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6	291	PEG, pH 6.0, VAPOR DIFFUSION, SITTING DROP, temperature 291K