4KSS
Crystal Structure of Vibrio cholerae ATPase GspsE Hexamer
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-01-31 |
| Detector | RIGAKU SATURN 944 |
| Wavelength(s) | 1.542 |
| Spacegroup name | P 42 21 2 |
| Unit cell lengths | 205.064, 205.064, 234.983 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 7.580 |
| R-factor | 0.34924 |
| Rwork | 0.349 |
| R-free | 0.35989 |
| RMSD bond length | 0.037 |
| RMSD bond angle | 1.723 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 90.000 | 90.000 | 7.250 |
| High resolution limit [Å] | 7.000 | 15.060 | 7.000 |
| Rmerge | 0.240 | 0.089 | |
| Number of reflections | 8382 | ||
| <I/σ(I)> | 3 | ||
| Completeness [%] | 99.3 | 94.9 | 100 |
| Redundancy | 6.7 | 6.1 | 6.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 9 | 277 | 7% PEG6000, 0.1M Bicine, 5mM ADP, 5mM MgCl2, 5 mM AMPPNP, pH 9, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
