4KSR

Crystal Structure of the Vibrio cholerae ATPase GspE Hexamer

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2012-12-06
Detector	DECTRIS PILATUS 6M
Spacegroup name	P 2 21 21
Unit cell lengths	112.467, 132.911, 142.750
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.720 - 4.200
R-factor	0.38393
Rwork	0.384
R-free	0.37624
RMSD bond length	0.038
RMSD bond angle	1.738
Data scaling software	XSCALE
Phasing software	PHASES
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	38.720	3.980
High resolution limit [Å]	3.780	3.780
Rmerge	0.106
Number of reflections	21927
<I/σ(I)>	12.2
Completeness [%]		96.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.1	277	0.1M BisTris, 5mM ADP, 5mM MgCl2, 5mM AlCl3, 15mM NaF, 16% PEG300, 0.2M ammonium sulfate, pH 6.1, VAPOR DIFFUSION, SITTING DROP, temperature 277K