4KSN
C-terminal domain of SdbC protein from Legionella pneumophila.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-04-18 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9792 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 90.240, 57.928, 81.448 |
| Unit cell angles | 90.00, 121.78, 90.00 |
Refinement procedure
| Resolution | 45.770 - 1.860 |
| R-factor | 0.18975 |
| Rwork | 0.188 |
| R-free | 0.22488 |
| Structure solution method | SAD |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.747 |
| Data reduction software | HKL-3000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHENIX |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 45.770 | 50.000 | 1.900 |
| High resolution limit [Å] | 1.860 | 5.070 | 1.870 |
| Rmerge | 0.065 | 0.047 | 0.629 |
| Number of reflections | 29977 | ||
| <I/σ(I)> | 11.9 | 1.99 | |
| Completeness [%] | 99.5 | 98 | 94.5 |
| Redundancy | 5.5 | 5.5 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 294 | 30% Tacsimate, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 294K |
