4KSK
Gumby/Fam105B in complex with ubiquitin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-08-20 |
| Detector | RIGAKU SATURN 944+ |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 84.273, 43.309, 113.918 |
| Unit cell angles | 90.00, 108.28, 90.00 |
Refinement procedure
| Resolution | 24.430 - 2.400 |
| R-factor | 0.21702 |
| Rwork | 0.214 |
| R-free | 0.26764 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4ksj |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.643 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 25.000 | 2.530 | 2.440 |
| High resolution limit [Å] | 2.400 | 2.490 | 2.400 |
| Rmerge | 0.161 | 0.619 | |
| Number of reflections | 28755 | ||
| <I/σ(I)> | 13.9 | 2.2 | |
| Completeness [%] | 96.7 | 93.3 | 76.1 |
| Redundancy | 5.8 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 298 | 100 mM Bis Tris, 200 mM AmSO4, 20% PEG3350, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
