4KRE
Structure of Human Argonaute-1 bound to endogenous Sf9 RNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-06-19 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.075 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 70.470, 98.630, 73.210 |
| Unit cell angles | 90.00, 110.96, 90.00 |
Refinement procedure
| Resolution | 32.926 - 1.754 |
| R-factor | 0.172 |
| Rwork | 0.171 |
| R-free | 0.20190 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.184 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.8.1_1168) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 1.800 |
| High resolution limit [Å] | 1.750 | 7.850 | 1.750 |
| Rmerge | 0.055 | 0.041 | 0.422 |
| Number of reflections | 91609 | 907 | 5855 |
| <I/σ(I)> | 12.56 | 25.79 | 1.96 |
| Completeness [%] | 98.0 | 82.5 | 85.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 9 | 291 | 13% PEG 3350, 9% iso-propanol, 0.1M Tris pH 9.0, vapor diffusion, hanging drop, temperature 291K, VAPOR DIFFUSION, HANGING DROP |
