4KR2
Glycyl-tRNA synthetase in complex with tRNA-Gly
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Detector technology | CCD |
| Collection date | 2012-11-01 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97930 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 135.964, 88.499, 80.497 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 32.252 - 3.292 |
| R-factor | 0.2376 |
| Rwork | 0.236 |
| R-free | 0.27710 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1GAX and 2PME |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.905 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((phenix.refine: 1.8_1069)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.420 |
| High resolution limit [Å] | 3.292 | 3.300 |
| Rmerge | 0.126 | 0.490 |
| Number of reflections | 13638 | |
| <I/σ(I)> | 10.4 | 5.63 |
| Completeness [%] | 89.4 | 95.2 |
| Redundancy | 4.3 | 4.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | 32% PEG600, 0.1M NaCl, 0.1M MES, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
