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4KQY

Bacillus subtilis yitJ S box/SAM-I riboswitch

Replaces:  3NPB
Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeSYNCHROTRON
Source detailsCHESS BEAMLINE F1
Synchrotron siteCHESS
BeamlineF1
Temperature [K]80
Detector technologyCCD
DetectorADSC QUANTUM 270
Wavelength(s)0.9
Spacegroup nameP 31 2 1
Unit cell lengths58.732, 58.732, 204.091
Unit cell angles90.00, 90.00, 120.00
Refinement procedure
Resolution24.680 - 3.020
R-factor0.24291
Rwork0.237
R-free0.30025
Structure solution methodMOLECULAR REPLACEMENT
RMSD bond length0.006
RMSD bond angle1.552
Data reduction softwareHKL-2000
Data scaling softwareHKL-2000
Phasing softwarePHASER
Refinement softwareREFMAC (5.7.0032)
Data quality characteristics
 Overall
Low resolution limit [Å]24.680
High resolution limit [Å]3.010
Number of reflections7365
Completeness [%]94.6
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1VAPOR DIFFUSION729840mM sodium cacodylate, pH 7.0, 80mM strontium chloride, 15% MPD, 2mM spermine, 1mM SAM, VAPOR DIFFUSION, temperature 298K

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PDB entries from 2024-05-15

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