4KQW
The structure of the Slackia exigua KARI in complex with NADP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL12-2 |
| Synchrotron site | SSRL |
| Beamline | BL12-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2011-07-01 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.0332 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 52.205, 118.934, 62.158 |
| Unit cell angles | 90.00, 101.17, 90.00 |
Refinement procedure
| Resolution | 60.980 - 1.390 |
| R-factor | 0.1609 |
| Rwork | 0.160 |
| R-free | 0.18660 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1np3 |
| RMSD bond length | 0.029 |
| RMSD bond angle | 2.744 |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 60.990 | 60.990 | 1.470 |
| High resolution limit [Å] | 1.390 | 4.410 | 1.390 |
| Rmerge | 0.039 | 0.024 | 0.318 |
| Number of reflections | 137256 | ||
| <I/σ(I)> | 12 | ||
| Completeness [%] | 92.9 | 91 | 78.9 |
| Redundancy | 2.6 | 2.9 | 2.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 297 | unbuffered 0.2M di-ammonium tartarate and 20% w/v PEG-3350, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 297K |
