4KQN
2.8 Angstrom Resolution Crystal Structure of D-Hydantoinase from Bacillus sp. AR9 in C2221 Space Group
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 298 |
| Detector technology | IMAGE PLATE |
| Collection date | 2006-02-14 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.5418 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 90.877, 185.239, 113.447 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 25.410 - 2.800 |
| R-factor | 0.20921 |
| Rwork | 0.206 |
| R-free | 0.26663 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1yny |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.614 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 25.410 |
| High resolution limit [Å] | 2.800 |
| Number of reflections | 19930 |
| Completeness [%] | 88.0 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 298 | 100mM Imidazole, 225mM CsCl, 15% PEG3350 , pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
