4KQC
ABC transporter, LacI family transcriptional regulator from Brachyspira murdochii
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-02-02 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9792 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 38.356, 59.241, 65.454 |
| Unit cell angles | 90.00, 102.90, 90.00 |
Refinement procedure
| Resolution | 31.900 - 1.620 |
| R-factor | 0.1529 |
| Rwork | 0.151 |
| R-free | 0.18830 |
| Structure solution method | SAD |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.694 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHENIX |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 31.900 | 50.000 | 1.650 |
| High resolution limit [Å] | 1.620 | 4.400 | 1.620 |
| Rmerge | 0.103 | 0.060 | 0.534 |
| Number of reflections | 35470 | ||
| <I/σ(I)> | 9.7 | 2.15 | |
| Completeness [%] | 97.1 | 97.9 | 81.1 |
| Redundancy | 3.4 | 3.4 | 2.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.3 | 277 | 0.2 M nitrate sulfate, 20% PEG-3350, pH 6.3, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
