4KQ0
Crystal structure of double-helical CGG-repetitive RNA 19mer complexed with RSS p19
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9834 |
| Spacegroup name | H 3 |
| Unit cell lengths | 90.926, 90.926, 147.791 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 15.000 - 2.100 |
| R-factor | 0.1818 |
| Rwork | 0.179 |
| R-free | 0.22700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.756 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | REFMAC |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 40.000 | 40.000 | 2.180 |
| High resolution limit [Å] | 2.100 | 4.520 | 2.100 |
| Rmerge | 0.061 | 0.051 | 0.293 |
| Number of reflections | 13256 | ||
| <I/σ(I)> | 16.6 | ||
| Completeness [%] | 94.5 | 96.1 | 69.8 |
| Redundancy | 3.1 | 3.1 | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 291 | 1.7 M ammonium sulfate, 0.1 M HEPES, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
