4KPR
Tetrameric form of rat selenoprotein thioredoxin reductase 1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-2 |
| Synchrotron site | ESRF |
| Beamline | ID23-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-06-17 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.8726 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 162.990, 162.990, 236.440 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 60.670 - 2.400 |
| R-factor | 0.1615 |
| Rwork | 0.160 |
| R-free | 0.18708 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3ean |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.816 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 70.000 | 2.530 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.094 | 0.670 |
| Number of reflections | 137497 | |
| <I/σ(I)> | 8.5 | 1.7 |
| Completeness [%] | 97.3 | 90 |
| Redundancy | 3.9 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 45 % MPD and 100 mM Tris-Cl buffer , pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
