4KP0
Crystal Structure of the human Chymase with TJK002
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
| Synchrotron site | Photon Factory |
| Beamline | AR-NW12A |
| Detector technology | CCD |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 56.920, 56.920, 177.490 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 41.000 - 2.800 |
| R-factor | 0.2415 |
| Rwork | 0.226 |
| R-free | 0.33020 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1klt |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.697 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.50109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.900 |
| High resolution limit [Å] | 2.800 | 2.800 |
| <I/σ(I)> | 30.3 | |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 15.2 | 13.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 293 | 100mM sodium citrate, 15% PEG1500, 20% 2-propanol, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
