4KNQ

Crystal structure of 1nt-5'-overhanging double-helical CCG-repetitive RNA 20mer complexed with RSS p19

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-4
Synchrotron site	ESRF
Beamline	ID14-4
Temperature [K]	100
Detector technology	CCD
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9395
Spacegroup name	H 3 2
Unit cell lengths	91.217, 91.217, 147.815
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	15.000 - 1.820
R-factor	0.1834
Rwork	0.182
R-free	0.20900
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.021
RMSD bond angle	1.995
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	REFMAC
Refinement software	REFMAC

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.890
High resolution limit [Å]	1.820	3.920	1.820
Rmerge	0.139	0.133	0.850
Number of reflections	21280
<I/σ(I)>	15.5
Completeness [%]	98.6	99.2	93.6
Redundancy	5.2	4.8	4.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	291	1.8 M ammonium sulfate, 0.1 M MES, pH 6, VAPOR DIFFUSION, HANGING DROP, temperature 291K