4KMY

Human folate receptor beta (FOLR2) at neutral pH

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 4.2.2
Synchrotron site	ALS
Beamline	4.2.2
Temperature [K]	100
Detector technology	CCD
Collection date	2011-03-02
Detector	NOIR-1
Wavelength(s)	1.0000
Spacegroup name	P 61
Unit cell lengths	75.026, 75.026, 97.188
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	37.513 - 1.795
R-factor	0.1649
Rwork	0.164
R-free	0.19330
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4kmz
RMSD bond length	0.009
RMSD bond angle	1.113
Data reduction software	DENZO
Data scaling software	SCALEPACK
Refinement software	PHENIX (1.7.1_743)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.830
High resolution limit [Å]	1.790	4.880	1.790
Rmerge	0.093	0.074	0.455
Number of reflections	28977
<I/σ(I)>	14.9
Completeness [%]	99.9	99.8	99.7
Redundancy	4.7	4.7	3.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.4	293	1.0 M Ammonium citrate tribasic, pH 7.0, 0.1 M Bis-Tris Propane, pH 7.0, Vapor diffusion, sitting drop, temperature 293K