4KMR
Structure of a putative transcriptional regulator of LacI family from Sanguibacter keddieii DSM 10542.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-G |
Synchrotron site | APS |
Beamline | 21-ID-G |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-04-23 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 0.97856 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 42.175, 120.205, 44.962 |
Unit cell angles | 90.00, 92.59, 90.00 |
Refinement procedure
Resolution | 22.470 - 1.450 |
R-factor | 0.12791 |
Rwork | 0.125 |
R-free | 0.17750 |
Structure solution method | SAD |
RMSD bond length | 0.015 |
RMSD bond angle | 1.832 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | HKL-3000 |
Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 1.480 |
High resolution limit [Å] | 1.450 | 1.450 |
Rmerge | 0.067 | 0.500 |
Number of reflections | 78844 | |
<I/σ(I)> | 33.9 | 3.7 |
Completeness [%] | 99.8 | 100 |
Redundancy | 3.7 | 3.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 0.2 M Magnesium Chloride, 0.1 M HEPES, 25% w/v PEG3350, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |