4KMG
Crystal structure of cytochrome c6B from Synechococcus sp. WH8102
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.2 |
| Synchrotron site | BESSY |
| Beamline | 14.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-12-15 |
| Detector | RAYONIX MX225HE |
| Wavelength(s) | 0.91841 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 106.110, 28.980, 24.680 |
| Unit cell angles | 90.00, 92.30, 90.00 |
Refinement procedure
| Resolution | 27.950 - 1.400 |
| R-factor | 0.12398 |
| Rwork | 0.120 |
| R-free | 0.18102 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4eif |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.769 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 27.950 | 1.490 |
| High resolution limit [Å] | 1.400 | 1.400 |
| Rmerge | 0.059 | 0.559 |
| Number of reflections | 14663 | |
| <I/σ(I)> | 13.8 | 2.02 |
| Completeness [%] | 97.9 | 90.1 |
| Redundancy | 3.6 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 292 | 2.2 M sodium malonate, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 292K |
