4KMC
Structure analysis of M. Tuberculosis rRNA transcriptional regulator CarD and its interaction with T. Aquaticus RNA polymerase-BETA1 domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-04-20 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.97625 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 60.957, 60.957, 59.478 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.479 - 2.150 |
| R-factor | 0.22 |
| Rwork | 0.218 |
| R-free | 0.25290 |
| Structure solution method | SAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.866 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.8.1_1168) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.300 | 2.190 |
| High resolution limit [Å] | 2.150 | 2.150 |
| Rmerge | 0.081 | 0.503 |
| Number of reflections | 6559 | |
| <I/σ(I)> | 10.3 | 7.9 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 27.4 | 28.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 278 | 16% PEG 4000, 0.2M Magnisium sulphate, 30% Ethylene Glycol, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 278K |
