4KMA
Crystal structure of Drosophila Suppressor of Fused
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-10-01 |
| Wavelength(s) | 1.000 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 71.824, 113.367, 131.147 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.680 |
| R-factor | 0.2502 |
| Rwork | 0.248 |
| R-free | 0.29710 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4km8 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.019 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.800 |
| High resolution limit [Å] | 2.680 | 5.810 | 2.700 |
| Rmerge | 0.137 | 0.087 | 0.387 |
| Number of reflections | 14179 | ||
| <I/σ(I)> | 9 | ||
| Completeness [%] | 92.4 | 99.6 | 100 |
| Redundancy | 9.2 | 9.1 | 9.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 277 | 16% PEG 3350, 0.2M Magnesium acetate, vapor diffusion, hanging drop, temperature 277K |
