4KL0
Crystal structure of the effector protein XOO4466
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-1A |
| Synchrotron site | Photon Factory |
| Beamline | BL-1A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-12-14 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 0.9789, 0.9791, 0.9639 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 47.832, 70.528, 49.083 |
| Unit cell angles | 90.00, 99.89, 90.00 |
Refinement procedure
| Resolution | 39.881 - 1.598 |
| R-factor | 0.2035 |
| Rwork | 0.202 |
| R-free | 0.23540 |
| Structure solution method | MAD |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.743 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | PHENIX (1.7.2_869) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.660 |
| High resolution limit [Å] | 1.598 | 1.600 |
| Rmerge | 0.082 | 0.717 |
| Number of reflections | 42434 | |
| <I/σ(I)> | 26.284 | 2.374 |
| Completeness [%] | 99.8 | 99.6 |
| Redundancy | 7.5 | 7.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 295 | 8.5% PEG 8000, 8.5% PEG 1000, 15% glycerol, 10mM calcium chloride, pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 295K |
