4KKV
Crystal structure of candida glabrata FMN adenylyltransferase D181A Mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E SUPERBRIGHT |
| Temperature [K] | 93 |
| Detector technology | IMAGE PLATE |
| Collection date | 2010-07-15 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.54178 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 80.257, 80.257, 78.169 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 24.400 - 1.740 |
| R-factor | 0.17354 |
| Rwork | 0.172 |
| R-free | 0.20140 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3fwk |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.082 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.770 |
| High resolution limit [Å] | 1.740 | 1.740 |
| Rmerge | 0.053 | 0.497 |
| Number of reflections | 30119 | |
| <I/σ(I)> | 21.6 | 1.26 |
| Completeness [%] | 99.3 | 95.4 |
| Redundancy | 5.5 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 289 | 0.1 M Sodium acetate, pH 5.0, 8% w/v PEG 4000, VAPOR DIFFUSION, HANGING DROP, temperature 289K |
